RP-HPLC Method for Simultaneous Estimation of Paracetamol and Ibuprofen in Tablets
نویسندگان
چکیده
A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Paracetamol and Ibuprofen. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5μ , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 260nm.Aceclofenac was used as an internal standard. The retention time of ibuprofen, paracetamol and aceclofenac was 2.48, 4.45 and 6.34 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation.This study aimed at developing and validating an HPLC method, being simple, accurate and selective, and the proposed method can be used for the estimation of these drugs in combined dosage forms
منابع مشابه
Simultaneous, Stability Indicating Method Development and Validation for Related Compounds of Ibuprofen and Paracetamol Tablets by RP-HPLC Method
A simple, precise, accurate, simultaneous and stability-indicating RPLC method developed with an effective resolution for active pharmaceutical ingredients and marketed drug products. This method effectively separate all the related substances of Ibuprofen and Paracetamol along with impurities. This method is using in the estimation assay of Ibuprofen and paracetamol in drug substance also. The...
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